NANO25 OFFERS RESEARCHERS UNIQUE
    APPROACH TO SERIAL DILUTIONS

Hudson Control Group's eight-channel nano25™ is the most useful nanoliter dispenser for broadcasting reagents on the market today, and will provide outstanding results for Serial Dilution Applications. Using the nano25™, serial dilution can be achieved by a direct dispense methodology, improving accuracy and greatly accelerating throughput.
Traditional methods for doing serial dilutions using conventional liquid handlers requires repeated dispensing, mixing and aspiration steps as the sample is added to the first then succeeding columns in successively greater dilutions, until the plate is full. Researchers can manually check a conventional system to validate it, but when researchers are running their plates, they have no way to know that, the correct volume was actually dispensed, or whether the mixing was sufficient to assure proper dilutions other than with internal calibration standards and spot checks. Any mishap in the process is then amplified down the line, resulting in wasted reagents, researcher's time, and money.

Using the nano25™, serial dilutions are prepared by a direct injection of the actual desired amount of reagent into each column. The nano25™ aspirates sufficient volume, and then multi dispenses the appropriate different volumes across each plate. The nano25™ can do this because each and every dispense is confirmed and because of its great dynamic range, from 10 uL to 25 nL per dispense. Since every dispense is separately controlled and measured, it is possible to dispense different volumes down the column. Each channel in the nano25™ offers independent pipetting control and liquid level detection, so that any combination of channels can be used at any one time. This capability provides researchers with tremendous application flexibility and speed, allowing them to perform one-step serial dilutions, normalization and multi-parameter experiments at very high throughput. The nano25™ takes out the guesswork on whether the correct dilution occurred and allows researchers to proceed with confidence.

Employing adaptive control and real-time feedback, the nano25™ robotic pipettor provides accurate, low-volume delivery of liquids across a dynamic range of three decades, starting at 25 nanoliters. The nano25™'s automated diagnostics detect and correct clogging, adapt to liquid temperature, and automatically correct for viscosity and liquid compressibility. In addition, it self-calibrates on every pipetting cycle and each of the eight tips is independently controlled and monitored for each dispense. The system lends itself to automation at a low cost and features a compact 19" x 22" (485mm x 550mm) footprint which easily fits on lab benchtops and into standard fume hoods.

The nano25™ can be loaded and operated manually, but is also fully compatible with robot loading systems from most manufacturers, including Hudson Control’ s own PlateCrane EX®. It can also be used in-line with the Hudson Control LabLinx® high-throughput track system for unparalleled high-throughput and ease of setup.

The nano25™ is powered by Hudson Control Group's SoftLinx™ software, which facilitates the robotic pipettor's integration with most robots and other automated instruments. If SoftLinx™ doesn't already include an interface to a specific lab instrument, the Hudson Control team will develop one.

SoftLinx™ will communicate with each instrument via a serial port from the computer, and oversee the process of moving labware among instruments, running those instruments' internal programs and then moving to the next instrument or an output stack. SoftLinx™ features an icon-based, drag-and-drop method editor, and uses VBA for instrument interfaces.